dCTCF aligns with Drosophila insulator-binding proteins SU(HW) and BEAF-32. (A) Venn diagram depicting overlap of dCTCF binding sites with those of BEAF-32 and SU(HW). Overlap represented as number of sites (summits ±150 bp) in which dCTCF intersects BEAF-32 and/or SU(HW) and target sequences are present for each insulator protein, suggesting close alignment (within 150 bp). (B) Example of ChIP-seq profile for dCTCF, BEAF-32, SU(HW), and CP190 on chromosome 3L; in which case dCTCF aligns with both BEAF-32 and SU(HW), where each cognate target is present. (C) Number of sites in which dCTCF, BEAF-32, and SU(HW) contain appropriate target sequences. Percentages of sites in which dCTCF, BEAF-32, and SU(HW) align closely with other DNA-binding insulator proteins. Most (90%) alignments include dCTCF, and as many as 40% of dCTCF sites align with either BEAF-32 and/or SU(HW).
