ONECUT1 ChIP-seq in heterozygous Rad21+/− mouse liver cells shows preferential loss of TF binding events where no motif is present. (A) Sample region near the BC031353 gene showing overall reduction in RAD21 ChIP signal in heterozygous Rad21+/− cells (responsive RAD21) and associated significant reduction in ONECUT1 ChIP signal within two CRMs (responsive ONECUT1). The ONECUT1 binding event overlapping the TSS contains no ONECUT1 motif. (B) WT ONECUT1 CRMs without motifs show a preferential decrease in ChIP signal (FDR < 0.1) in heterozygous Rad21+/− mouse liver cells (Fisher's exact test P = 10−4). Regions of interest (ROI) are those CRMs where RAD21 binding was ablated in heterozygous Rad21+/− mouse liver cells (responsive RAD21).
