Many noncanonical poly(A) sites in the pre-MZT embryo. (A) Abundance of sample-specific poly(A) sites. Site classification was as described in Supplemental Figure S1. Downstream sites are those appearing up to 8 kb downstream from the annotated 3′ ends but without the support for connectivity with the stop codon required for assignment as a novel 3′ UTR. (B) Sequence composition near poly(A) sites specific to the testis. (C) Poly(A) sites of edc3. The 3′ UTRs shown are as annotated in Ensembl v66. (D) Density of poly(A) sites occurring within 3′ UTRs from the indicated samples. Poly(A)-site density was defined as the ratio between the number of poly(A) sites and the length of the longest 3′ UTR. (E) Densities of poly(A) sites in the coding sequence and 3′ UTRs plotted for genes expressed in the pre-MZT embryo. (F) Sequence composition near poly(A) sites specific to the pre-MZT embryo.
