Ultrashort and progressive 4sU-tagging reveals key characteristics of RNA processing at nucleotide resolution

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Figure 5.
Figure 5.

Processing of the SNORD116 cluster. (A) Read density for the SNORD116 cluster region (overview). (B) Read density plot for a typical snoRNA of the SNORD116 cluster only shows reads at the 5′ end of the mature snoRNA, but no intronic sequences, thus representing sequencing of the mature, unfragmented snoRNA. (C) Read density plot for a typical intronic snoRNA. Here, intronic sequences were observed until 60-min 4sU-RNA.

This Article

  1. Published in Advance April 26, 2012, doi: 10.1101/gr.131847.111 Genome Res. 22: 2031-2042

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