Moderate EZH2 sites are noncanonical PRC2 targets. (A) Characteristics of moderate EZH2 sites on d0 and d7. The portion of sites overlapping CpG islands is indicated by stripes. (B) Allelic skewing of moderate EZH2 sites. Numbers of sites skewing to Cast (blue) or 129 (red) are shown. Those with significant skewing (P < 0.05, norm. approx. of binomial) are shown in darker blue and red. (Gray bars) nonpolymorphic sites; (n/d) not determined. (C) Changes in EZH2 and H3K27me3 densities between d0 and d7. Medians (turquoise, Chr13; orange, ChrX), 25–75 percentile (box) and 10–90 percentile (error bars) are shown. (D) Metasite analysis for strong and moderate sites at indicated time points. Coverages were averaged over all strong (ChrX: 56, 147, 50 sites; Chr13: 79, 81, 83 sites for d0, d7, and MEFs) and moderate sites (ChrX: 1618, 4077, 1211 sites; chr13: 1241, 1041, 758 sites for d0, d7, and MEFs) and scaled from start to end. Profiles extend ±20 kb into flanks. Densities were normalized to the average site coverage over the chromosome.
