Many strong EZH2 sites are defined bivalent domains. (A) Characteristics of constant, acquired, and lost strong EZH2 sites on d0 and d7. The portion of sites overlapping CpG islands is indicated by stripes. (B) Changes in peak widths and densities (coverage per bp) for EZH2 and H3K27me3 between d0 and d7. Medians (turquoise, Chr13; orange, ChrX), 25–75 percentile (box) and 10–90 percentile (error bars) are shown. (C) Allelic skewing of strong EZH2 sites. Numbers of sites skewing to Cast (blue) or 129 (red) are shown. Those with significant skewing (P < 0.05, norm. approx. of binomial) are shown in darker blue and red. (Gray bars) Nonpolymorphic sites; (n/d), not determined. (D) Summed coverage of EZH2 and H3K27me3 at strong and moderate sites as a percentage of total chromosomal coverage on d0 and d7 (left) and as fold-change (d7/d0, right).
