SuUR mutant follicle cell amplicons exhibit enhanced replication fork progression. (A) aCGH of Oregon-R (OrR) and SuUR mutant FACS sorted 16C follicle cell nuclei at cytological locations encompassing Drosophila amplicon in follicle cells DAFC-66D and DAFC-7F. (B) SuUR mutant forks travel farther in the same developmental time window at DAFC-66D. SuUR mutant replication fork progression occurs between stage 11 and 13 in egg chamber development and is not due to alterations in egg chamber development timing. Quantitative PCR with primers 20 kb apart along the DAFC-66D amplicon was performed on hand-sorted egg chambers, relative to an unamplified control locus. The distances are from the peak of amplification (0 point). The experiment was performed in triplicate.
