Loss of SuUR function restores genome replication without restoring ORC binding or transcription, but does lead to depletion of the repressive H3K27me3 mark. (A,B) aCGH of proximal half of chromosome 2L in OrR (A) and SuUR mutant (B) third-instar salivary glands, as described in Figure 1A. (C) ChIP-seq peaks of ORC in SuUR mutant third-instar salivary glands, called as described in Figure 2. (D) The ChIP-seq peaks of ORC from wild type for comparison. (E) Number of reads from Illumina RNA sequencing of SuUR mutant third-instar salivary glands. (F) RNA Pol II ChIP of SuUR mutant third-instar salivary glands, normalized to IgG control ChIP. (G) H3K27me3 ChIP-chip in SuUR mutant third-instar salivary glands, normalized to input DNA.
