Figure 2.
Under-replicated regions are restrictive for ORC2 and RNA Pol II binding and are marked by weak binding of H3K27me3. (A) aCGH of proximal half of chromosome 2L, as described in Figure 1A. (B–E) RNA-seq, RNA Pol II ChIP-chip, H3K27me3 ChIP-chip, and ORC2 ChIP-seq peaks in Oregon-R (OrR) third-instar salivary glands, respectively. Total Illumina RNA sequencing reads are plotted; RNA Pol II ChIP was normalized to IgG control ChIP, and H3K27me3 ChIP data to input DNA. ORC2 ChIP-seq peaks were called by SPP compared with input sequencing lane; peaks for these data are organized downward by the UCSC Genome Browser.
