High DNase I sensitivity of the DH sites associated rice genes that differentially expressed in the seedling and callus. (A) Heatmap of DNase I sensitivity surrounding the TSSs of genes. Genes were sorted out based on fold change of gene expression of seedling relative to callus. The genes in the two panels are in the same order. The genes above/under the black lines were significantly up-regulated in the seedling and callus, respectively. DNase I sensitivity was indicated by DNase-seq read number per bp genome per million reads. (B) Percentage of differentially expressed genes associated with DNase I sensitivity changes between the seedling and callus in their genic region, 1000-bp and 200-bp upstream region of TSS, 200 bp downstream from the gene, exon, and intron. We used similar expressed genes and genes randomly selected (10 times) as a control, with the same sample size of differentially expressed genes.
