Figure 3.
Sequence motif analyses at tagmentation and Illumina fragmentation sites. Genomic sequences at 1000 randomly chosen sites of transpososome integration (top; single enzyme, AcuI 10-pg input tagmentation library) or physical sonication induced shearing positions (bottom; Illumina library) were analyzed for over-represented sequence motifs and plotted by nucleotide prevalence at each base position. The fragmentation site is shown at base +1. A weak sequence preference spanning an ∼13-bp region was found in the tagmented library. No sequence preference was found in the Illumina library.
