Synthetic spike-in standards for RNA-seq experiments

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 4.
Figure 4.

Stereotypic read density heterogeneity in ERCC RNA-seq. (A) Traces of relative coverage along ERCC-0002 in two different ENCODE libraries (libraries 7, 8). The pattern is highly reproducible (Pearson's r = 0.96). (B) Average relative coverage along all control RNAs for ERCC spiked in the H. sapiens libraries (libraries 7–50). Dashed lines represent 1 SD around the average across different libraries.

This Article

  1. Published in Advance August 4, 2011, doi: 10.1101/gr.121095.111 Genome Res. 21: 1543-1551

Preprint Server



Current Issue

  1. January 2026, 36 (1)
  1. Current Issue
  1. Alert me to new issues of Genome Research