Synthetic spike-in standards for RNA-seq experiments

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Figure 2.
Figure 2.

Estimation of cellular transcript abundance in a S2 cell. (A) This plot shows results from a library (library 3) made of 100 ng S2 polyA+ RNA (mRNA yield for this extraction is 0.175 pg/cell) and 5 ng of pool 15 ERCC RNAs. A linear regression of abundance estimated from RNA-seq and the known input amounts. Dashed lines represent 95% confidence intervals for the regression fit. (B) Distribution of S2 transcript abundance estimated from RNA-seq.

This Article

  1. Published in Advance August 4, 2011, doi: 10.1101/gr.121095.111 Genome Res. 21: 1543-1551

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