Replication origins in metazoans are linked to expressed genes. (A) Replication origins are significantly associated with transcribed genes (*: P < 0.001) in both mouse MEF and Drosophila Kc cells. (B) Association of replication origins with gene partitions in MEFs (left panel) and Drosophila Kc cells (right panel). Replication origins are found more frequently at gene promoters (mouse cells) and exonic sequences (mouse and Drosophila cells, *: P < 0.001). (C) Distribution of mouse replication origins along a gene. The position of each origin is allocated depending on the length of the gene adjusted to 100%. (D) Nascent strand signal strength at TSS in ES cells and (E) Drosophila Kc cells. The enrichment value is the log10 of the combined P-value associated with NS signal (see Supplementary Information). (F) NS signals in mouse ES cells are associated with CGI-positive TSS but not with CGI-negative TSS.
