ORF target predictions recover functional targets for additional miRNAs. (A) miR-23a-mediated repression of reporters with miR-23 ORF sites. Reporters were constructed and assayed as in Figure 1E, except miR-23a was the cognate miRNA and miR-181a was the noncognate miRNA (n = 12; p < 10−6). (B) miR-199a-mediated repression of a reporter with miR-199 ORF sites, otherwise as in A (n = 12; p = 2 × 10−6 and 0.08, for ZNF791 and ZNF20, respectively). (C) miR-370-mediated repression of reporters with miR-370 ORF sites. miR-124 was the noncognate miRNA, otherwise as in A (n = 15; p = 0.0009 and 10−8, for HDAC5 and IVL, respectively). (D) Direct miR-370-mediated repression of an IVL reporter. Repression was calculated and depicted as in A, additionally normalizing repression of the reporter with wild-type sites (WT) to that of a reporter in which the ORF sites were mutated (n = 12; p < 10−4).
