Figure 2.
pax6 knockdown in zebrafish results in reduced size, particularly of nervous system structures. (A) Representative control sibling. Knockdown with morpholino oligonucleotides at 1–2-cell stages of (B) pax6a, (C) pax6b, and (D) both pax6a and pax6b genes leading to a phenotype with smaller eyes and reduced central nervous system structures, e.g., neural tube, in comparison to control siblings The abnormal phenotype is present from early stages (24 hpf), and persists long after 48 hpf (B–D). Treated embryos die before day 4.
