Figure 5.
Sense-antisense HeliScopeCAGE signal. (A) Distribution of CAGE tag signal on the genome relative to 100 bp upstream, 5′ UTR, internal exons, 3′ UTR, and introns in HeliScope CAGE libraries from 5 μg of THP-1, 5 μg of HeLa, and 5 μg of THP-1 when the reverse transcription is carried out in the presence of actinomycin D. (B) Genomic view of the ACTB locus. (i) Linear scale 5 μg of THP-1; (ii) log scale 5 μg of THP-1; (iii–v) log scale 5 μg of THP-1 in the presence of 0.1, 0.2, or 0.4 mg/mL actinomycin D demonstrating sense and antisense painting of exons visually defining the gene boundaries (log scale).
