Defining the relative specificity of PluCR oligonucleotides for POU2F1 (Oct1) and POU5F1 (Oct4). (A) The enrichment of all 40,000 oligonucleotides in the bound fraction of POU5F1 (Oct4) and POU2F1 (Oct1) was compared by scatterplot. The top 1% of oligonucleotides defined in each experiment is indicated by vertical (POU2F1) and horizontal (POU5F1) dashed lines. Motifs derived from the doubly enriched and POU specific fraction were identified as in Figure 3. Their enrichment in the bolded data points is printed to the right of the motifs. (B) The association between TRANSFAC/JASPER binding models and POU2F1/POU5F1 binding tendencies. The entire set of vertebrate TRANSFAC/JASPER binding models was used to annotate the oligonucleotide pool. The average POU2F1 (Oct1; x-axis) and POU5F1 (Oct4; y-axis) binding enrichment of the set of oligonucleotides that contain a significant match to a TRANSFAC motif was plotted for each TRANSFAC motif that contained multiple matches.
