
Number of junctions, transcripts, and genes detected at different sequencing depths. The numbers of genes, transcripts, and junctions detected in our 879-million-read data set were assumed to be the “final” values. Then, the percentages of these “final” values detected at various sequencing depths were determined. For example, with 100 million reads, 76% of the junctions, 90% of transcripts, and 81% of genes were detected.











