Example plots in Artemis of the four different loss-of-fitness patterns illustrated in Figure 2; mRNA ablation is associated with a defect in all four induced conditions (blue), a more pronounced defect in bloodstream form cells (red) or procyclic cells (green), or a differentiation defect (purple). Cells carrying RNAi target fragments that negatively impact fitness through dsRNA expression and RNAi-mediated ablation are relatively depleted as the population expands and these changes are reported by the depth of sequence coverage relative to the uninduced control (no Tet). Each peak represents a unique RNAi target fragment. The genes shown encode a dynein heavy chain (Tb927.4.870), an intraflagellar transport protein (Tb927.10.14470), dihydrolipoamide acetyltransferase (Tb927.10.7570), and an uncharacterized zinc ion binding protein (Tb09.160.265).
