Alu insertions predicted in the Yoruban parent–child trio. (A) Novel Alu integration sites for four chromosomes are shown. Alu sequences transmitted from the father (green) are compared with those transmitted from the mother (red). Purple bars represent centromeres and black bars denote Alu insertions shared by both parents and transmitted to the offspring. In these chromosomes, five de novo insertions were predicted in the child (blue), and further marked with a red arrow. Note that the predicted de novo Alu insertion in distal location in chromosome Y is very close to a transmitted integration, and it is difficult to see at this resolution. (B) PCR validation results for three individuals for four different loci (GM18507_Alu5, GM18507_Alu7, GM18507_Alu9 and GM18508_Alu5).
