Figure 1.
Distinct TCRB sequences. (A) Sequence coverage of distinct TRBJ sequences observed upon alignment of Illumina reads quality filtered to >99.9% predicted accuracy (Q30). There was substantial coverage, up to several million-fold, of the 13 human TRBJ segments, but thousands of artifactual distinct TRBJ sequences were also observed, arising from residual sequencing error. (B) Restricting the data set to D96 effectively retains all real TRBJ sequences and excludes all of the artifactual TRBJ sequences observed when no coverage restriction is applied (D100).
