Shotgun proteomics aids discovery of novel protein-coding genes, alternative splicing, and “resurrected” pseudogenes in the mouse genome

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Figure 9.
Figure 9.

(A) Two canonically splicing MS PSMs support the annotation of coding isoforms of a mouse Ppia processed pseudogene locus (OTTMUST00000018507). (B) Clustal alignment of parent PPIA protein (SWISS-PROT P17742), translations of both coding isoforms of the mouse Ppia processed pseudogene, and the putative translation of the pseudogene object. Residues of the coding isoforms that are covered by MS evidence are highlighted yellow. Residues of the parent polypeptide that are part of known domains are shown by colored boxes above the alignment.

This Article

  1. Genome Res. 21: 756-767

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