Shotgun proteomics aids discovery of novel protein-coding genes, alternative splicing, and “resurrected” pseudogenes in the mouse genome

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Figure 6.
Figure 6.

(A) Species-specific EST and peptide evidence supports the annotation of a protein-coding object at this locus. (B) Clustal alignment of the polypeptide sequences of this novel single-exon object with the orthologous objects from the rat and human genomes, respectively. There is no transcriptional support for either the rat or human model; however, the CDS frame is highly conserved and intact. The residues of the mouse translation that are covered by PSMs are colored yellow.

This Article

  1. Genome Res. 21: 756-767

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