Figure 3.
Multiplexing experiments are reproducible and productive, and the Calling Card–seq method performs well when the TF–Sir4 is expressed from its native genomic locus. (A) The number of independent Calling Card insertions within each promoter is plotted for two biological replicate experiments multiplexing eight TFs. Data for Yrm1 and Sef1 are shown here. (B) Sequence logos for newly discovered TF binding site motifs.
