Genes with opposite transcriptional responses to GR colocalize with Lcn2. (A) GR induced (underlined) and repressed (not underlined) genes from Affymetrix exon expression array analysis, located within Lcn2 contact regions before and after 1 h of hormone treatment. First, all genes in the Lcn2 contact regions were obtained by retrieving genes whose TSS is located within 10 kb of 4C positive probes (excluding the bait region). Genes were considered GR-responsive if their expression changed by more than twofold after Dex treatment. (B) Time-course analysis by qRT-PCR of nascent transcript levels of GR-regulated genes located within Lcn2 contact regions. Samples were collected before Dex treatment and 0.5, 1, 2, and 4 h after Dex induction. Results shown are the average of three independent experiments, with SD presented as error bars.
