P. deltoides small protein-coding candidate genes enriched from transcription units. (A) Number of genes in different sORF candidate subsets. (B) Proportion of the sORF subsets having known protein domains detected by InterProScan. Subset A contains the sORF candidates with high protein-coding potential predicted using known proteins as training sequences. Subset B contains sORF candidates conserved between P. deltoides and at least one other plant species. Subset C contains sORF candidates clustered into families. (Initial) The initial sORF candidate set (Fig. 1). (AB) The intersection of Subsets A and B. (ABC) (i.e., the high-confidence sORF candidate set) The intersection of Subsets A, B, and C. The value in parentheses represents the number of sORFs in each individual subset.
