Figure 1.
(A) Schematic of BRISK technique. (B) Representative ethidium bromide-stained agarose gel of BRISK products. (Lane 1) 1 kb DNA ladder; (lane 2) 100 bp DNA ladder; (lane 3) PCR amplification of BRISK fragments following ligation of asymmetric adaptors; (lane 4) amplification of unbound material from biotin column; (lane 5) amplification of beads following melt and elution of single-stranded DNA; (lane 6) ampification of material eluted from beads (desired product containing one long and one short adaptor); (lane 7) negative PCR control.
