Overview of whole genome profiling technology. (A) BAC library: BAC clones are available in a 384-wells plate format. (B) BAC pooling and DNA extraction: DNA is extracted after pooling each row (24 BACs) and each column (16 BACs). (C) Template preparation and sequencing: Pooled BAC DNA is digested (EcoRI/MseI) and amplified after barcoded adapters are ligated for pool identification after sequencing on the Illumina GA platform. (D) Deconvolution: Sequence tags (30–50 per BAC) are assigned to individual BACs based on presence in one row and one column pool. (E) Contiging: Overlapping (sets of) sequence tags from individual BAC clones generate contigs. Together, these contigs represent a sequence-based physical map of the genome.
