Validation and functional characterization of on-target TORC1–S6K pathway regulators using secondary screening for multiple phenotypes. On-target hits from the primary screen were screened in several validation and secondary screens on microarrays, as described in the Methods. The results were subjected to hierarchical clustering, and several notable clusters are marked. Note that synthetic experiments (experiments where the cell population was treated with a dsRNA in addition to the dsRNA spots on the array) were performed by transfecting cells with the indicated dsRNA and transfection reagent prior to seeding on microarrays, such that culture conditions were different from nonsynthetic experiments. See Methods for details.
