B1-X35S establishes a heterochromatic epigenetic mark that responds to AHR. The region from 1000 bp upstream (−1000) to 1500 bp downstream (+1500) from B1-X35S was analyzed for chromatin marks by qChIP in Hepa-1 cells with or without transfection of AHR+SLUG. (A) Location of B1-X35S in the promoter region of Dad1, Lpp, and Cabin1 and of the PCR fragments analyzed is indicated. (B) H3K9me3 status. (C) H3K27me3 status. Data were normalized to the amount of H3 immunoprecipitated. (D) In vivo binding of CTCF and PARP1 to B1-X35S was analyzed by qChIP in Hepa-1 cells with or without transfection of AHR+SLUG. PARP1 inhibitor 3-amino benzamide (3-ABA, 5 mM) was added for 24 h where indicated. Average results for genes Dad1, Lpp, Cabin1, Tbc1d1, and Rtl1 are shown. Experiments were performed in Hepa-1 cells and data are shown as mean ±SD.
