Dioxin receptor and SLUG transcription factors regulate the insulator activity of B1 SINE retrotransposons via an RNA polymerase switch

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Figure 3.
Figure 3.

Pol III binds B1-X35S: role of AHR and SLUG. (A) In vitro binding of Pol III and TFIIIC to B1-X35S, B1-Xmut35S, B1-X35Smut, or B1-Xmut35Smut was analyzed by DNA-binding affinity assays. (B) qChIP to address whether AHR and SLUG expression block Pol III recruitment to B1-X35S in vivo after transient transfection of Hepa-1 cells with AHR alone or with SLUG. (C,D) AHR was expressed with or without SLUG and their effects on histone H3 binding to B1-X35S (C) or Pol III binding to tRNALeu genes (D) determined by qChIP. (B,C) Average data from five B1-X35S-containing genes (Dad1, Lpp, Cabin1, Tbc1d1, and Rtl1). Data are shown as mean ±SD.

This Article

  1. Published in Advance February 3, 2011, doi: 10.1101/gr.111203.110 Genome Res. 21: 422-432

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