Figure 2.
B1-X35S has insulator activity in zebrafish. (A) B1-X35S and mutant B1Xmut35Smut in the enhancer+promoter-driven GFP vector, as illustrated, were microinjected into one-cell zebrafish embryos. Each animal was photographed and GFP signals quantified in CNS and somites. Measurements of individual zebrafish are shown in Supplemental Figure 2. (B) Insulator activity was calculated as the median GFP signal in muscle/CNS for each experimental condition. Data are shown as mean ±SD.
