Figure 3.
5′ variability of Drosophila canonical miRNAs. These charts summarize data for 135 canonical miRNAs that generated more than 1000 reads and had exclusively unique genomic mappings. (A,B) The 5′ end precision of mature miRNA species was generally high for well-expressed species; however, select loci generated abundant secondary and/or tertiary 5′ isomiRs. (C,D) The 5′ end precision of miRNA* (star) species was less than for mature miRNAs; still, only a relatively select group of highly expressed star species exhibited abundant 5′ isomiRs. The full analysis is available in Supplemental Table S4.
