PRKCA and coatomer play differential roles in transport of endogenous YES1 and other SH4 proteins. (A) Stable HeLa cells expressing SH4-HASPB-mCherry were transfected with a scrambled siRNA (a–c), and siRNAs directed against COPB1 (d–f) and PRKCA (g–i), and induced for reporter protein expression. Forty-eight hours post-transfection, cells were fixed, subjected to immunostaining against endogenous YES1, and subsequently analyzed by confocal microscopy. (B) Stable HeLa cells expressing fusion proteins of the SH4 domains of the acylated SRC kinases LCK, SRC, and FYN were transfected with a scrambled siRNA (a–c), and siRNAs directed against COPB1 (d–f) and PRKCA (g–i). Forty-eight hours post-transfection, cells were analyzed by live-cell confocal microscopy to determine the subcellular localization of the respective SH4-protein.
