Figure 5.
Validation of mass spectrometry-derived data using RT-PCR and cDNA sequencing. RT-PCR products were sequenced on both strands, and the resulting sequences were submitted to GenBank. GenBank accession numbers are indicated above each lane. (A) RT-PCR validation of 35 novel genes. (B) RT-PCR validation of 70 gene models that led to the correction of existing VectorBase gene annotations in genebuild AgambP3.4. The genes that belong to novel categories with respect to genebuild AgambP3.6 are marked with an asterisk (*).
