Deletion of ribosomal proteins causes accumulation of lysine and a lysine-related molecule. (A) Traces of rps19a and rps19b mutants, compared with the trace of the parental strain. The two ribosomal protein mutant strains show significant accumulation, exceeding that observed in slow growers alone, of both lysine and a lysine-related molecule compared with the parent strain. (To generate these data, we regrew and made fresh extracts of these strains, as the rps19a mutant seemed to have a suppressor mutation in the original set, as has been observed previously [Steffen et al. 2008].) (B) Hierarchical clustering of all strains reveals a group of genes, enriched for ribosomal proteins, with accumulation of lysine and the lysine-related metabolite. The rps19b mutant is shown separately as it does not cluster with this group but shows a similar pattern.
