Figure 1.
Canonical trace from a S. cerevisiae extract. Following overnight growth in synthetic complete media, yeast cultures were extracted with cold methanol. The extract was labeled with NBD-F, which fluorescently labels amine groups, and separated by capillary electrophoresis. The separation occurs in less than 10 min. Peaks were assigned by spike-in experiments and are labeled 1–18, corresponding to the compounds shown. Intensity, measured in relative fluorescent units, correlates with relative amino acid concentration. (Lysine has two peaks as it has two reactive amine groups and therefore can be labeled once or twice.)
