Figure 3.
Gene addition to the AAVS1 locus in hESCs using ZFNs. (A) Schematic overview depicting the editing strategy for both alleles of the PPP1R12C gene. Donor plasmids used to target the locus are shown above; gene elements are represented as in Figure 1B. (Puro) Puromycin resistance gene, (CAGGS) constitutively active CAGGS promoter, (M2rtTA) tetracycline reverse transactivator. (B) Still images from a time lapse movie (see Supplemental movie M1) imaging H2B-eGFP in a representative BGO1 cell targeted with the AAVS1 donor plasmids as shown in A. Scale bar, 10 μm.
