Figure 2.
Stability of GFP expression in K562 cells when driven by the endogenous PPP1R12C promoter. Shown is the mean fluorescence intensity of a GFP-positive cell pool (green X's), a clone derived by limiting dilution that is monoallelic at AAVS1 for the GFP ORF (blue squares), diallelic (yellow triangles), and negative control cells (black diamonds) measured over 25 d—∼30 cell doublings—of growth in nonselective medium. After 25 more days of passaging, the MFI remained essentially unchanged (VM Choi and EA Moehle, data not shown).
