A genome-wide map of human genetic interactions inferred from radiation hybrid genotypes
- 1 Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA;
- 2 Signal and Image Processing Institute, University of Southern California, Los Angeles, California 90089, USA
Abstract
Using radiation hybrid genotyping data, 99% of all possible gene pairs across the mammalian genome were tested for interactions based on co-retention frequencies higher (attraction) or lower (repulsion) than chance. Gene interaction networks constructed from six independent data sets overlapped strongly. Combining the data sets resulted in a network of more than seven million interactions, almost all attractive. This network overlapped with protein–protein interaction networks on multiple measures and also confirmed the relationship between essentiality and centrality. In contrast to other biological networks, the radiation hybrid network did not show a scale-free distribution of connectivity but was Gaussian-like, suggesting a closer approach to saturation. The radiation hybrid (RH) network constitutes a platform for understanding the systems biology of the mammalian cell.
Footnotes
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↵3 Corresponding author.
E-mail DSmith{at}mednet.ucla.edu; fax (310) 825-6267.
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[Supplemental material is available online at http://www.genome.org.]
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Article published online before print. Article and publication date are at http://www.genome.org/cgi/doi/10.1101/gr.104216.109.
- Copyright © 2010 by Cold Spring Harbor Laboratory Press
