Genome-wide identification of TAL1's functional targets: Insights into its mechanisms of action in primary erythroid cells

DNA motifs underlying the TAL1 peaks. (A) Logos representing the motifs identified in the sequences underlying the TAL1 peaks using de novo Weeder and Meme searches. (B) Real-time PCR analysis of anti-TAL1 and anti-GATA1 ChIP on selected loci. Chromatin derived from Ter119^- populations from Tal1^WT/WT and Tal1^RER/RER fetal liver culture cells was immunoprecipitated using anti-TAL1 and anti-GATA1 antibodies and the loci indicated on the graph analyzed by real-time PCR. The y-axis represents the enrichment over input DNA, normalized to a control sequence in the Gapdh gene. Error bars, ±1 SD from at least three independent experiments (*P < 0.01). Below the graph are shown the categories the genes belong to, as detected by ChIP-seq.