Genome-wide identification of TAL1's functional targets: Insights into its mechanisms of action in primary erythroid cells

Profile of TAL1 binding on chosen loci. (A) Selected known functional or novel genomic targets of TAL1 are represented. For each locus are shown (from top to bottom): the RefSeq annotation of the gene or part of the gene (orange, exons; thin lines, introns; arrow, position of the TSS); the ChIP-seq profiles in Ter119^- populations from Tal1^WT/WT (black tracks) and Tal1^RER/RER (red tracks) fetal liver cells. (B) Real-time PCR analysis of anti-TAL1 ChIP on selected loci. Chromatin derived from Ter119^- populations from Tal1^WT/WT and Tal1^RER/RER fetal liver culture cells was immunoprecipitated using anti-TAL1 antibodies and the loci indicated on the graph analyzed by real-time PCR. The y-axis represents the enrichment over input DNA, normalized to a control sequence in the Gapdh gene. N, negative control. Error bars, ±1 SD, from at least three independent experiments (*P < 0.01). Below the graph are shown the categories the peaks belong to, as detected by ChIP-seq.