Expression of Drosophila Her, CG16833, and aphid Her. Drosophila embryos stained for Her or CG16833 RNA (blue) using in situ hybridization. Scale bars, 50 μm for panels A–E and J–N. Embryos are oriented anterior to the left, dorsal top. (A–C) Expression of Her RNA in Drosophila embryos detected using in situ hybridization. Her RNA in a stage 5 (A), stage 11 (B), and stage 12 (C) embryo. At all stages Her RNA is ubiquitously distributed. (D,E) Sense controls for Her in situ hybridization stained under the same conditions as A–C. Weak staining is seen in early embryos, up to stage 4 (D); later embryos (E, stage 11) show no significant expression. (F–H) Viviparous aphid embryos stained for Her RNA (ACYPI009737) (blue) using in situ hybridization. All embryos are oriented with anterior to the left. Scale bars, 200 μm for panels F–I. Embryos are staged according to the scheme of Miura et al. (2003) and Chang et al. (2007). (F) At stages 11 and 12 (lateral view) Ap-Her is expressed diffusely throughout the embryonic germband during germband elongation including the presumptive head region. (G) By stage 13 and 14 (ventral view) limb buds are formed and diffuse Ap-Her staining is observed in the head region, with stronger staining observed in cells of the ventral midline as well as some surrounding cells. Expression of Ap-Her is not detected in the fully differentiated CNS. (H) At stage 18 (ventral view) expression of Ap-Her is restricted to tissues associated with the differentiating compound eyes. (I) Ap-otd (also known as oc) expression is shown for comparison. At stage 14 (ventral view) Ap-otd is strongly expressed in the presumptive cephalic regions of the head and in cells of the ventral midline in the developing CNS (Huang et al. 2010). (J–L) Expression of Drosophila CG16833 RNA detected by in situ hybridization. Expression is ubiquitous in all embryos, shown are stage 4 (J), stage 5 (K), and stage 12 (L). (M,N) Sense negative control in situ hybridization for CG16833. No staining is seen at any stage; shown are stage 6 (M, dorsal view) and stage 12 (N).
