Delayed replication timing of chromosome 4 after knockdown of HP1. Replication timing profiles of control (red, ctrl) and HP1 knockdown (blue, HP1 kd) Kc cells for a representative region on chromosome 4. X-axis, chromosomal position in base pairs; y-axis, log2 (early/late replication). Background coloring denotes regions that replicate earlier in HP1 kd cells (L:E, pink), regions that replicate earlier in control cells (E:L, blue), and regions replicating similarly in both cell types (white). Annotated genes are displayed below the profile (boxes, exons; lines, introns; small boxes, UTRs) and colored by their expression status (for details, see Methods; green, expressed in control and HP1 kd cells; blue, expressed only in HP1 kd cells; red, expressed only in control cells; gray, not expressed in control and HP1 kd cells). Transcription levels of control (red) and HP1 kd (blue) cells measured by tiling arrays are displayed on the same scale below, including transcription level differences (black). The direction toward the centromere (cen) is marked by an arrow.
