Deep RNA sequencing at single base-pair resolution reveals high complexity of the rice transcriptome

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 5.
Figure 5.

Gene fusions in rice transcriptome. The schematics describe the three types of transcript fusion events that were supported by paired-end linkage and junction reads. The black curve represents the paired-end reads that spanned the two genes. The exons of original genes are shown in colored bars connected by black lines. The sequences spanning the fusion junctions are indicated by the partition in the short reads. Hexanucleotide ESEs are denoted by vertical lines in the exon bars, and the same types of ESEs are the same color. For qRT-PCR validation of fusion events, the y-axis is the ΔCt values compared with ACTB gene. (A) A fusion event that occurred between two interchromosomal genes; (B) a fusion event that occurred between two neighboring genes; (C) a fusion event that occurred between two distant genes in the same chromosome.

This Article

  1. Genome Res. 20: 646-654

Preprint Server