Analysis of novel transcript units. (A) RNA-seq has identified a protein-coding gene that is absent in previous annotation. (Red bars) Coding regions; (gray bars) noncoding regions. (B) A 236-bp region in chromosome 2 that encompasses a novel microRNA gene. This microRNA gene was confirmed by deep sequencing of small RNA samples, and the existence of its mature microRNA was supported by abundant short reads. The secondary structure of this microRNA precursor is depicted below the sequence alignment. (C) The length distribution of newly identified transcripts. (D) A comparison of expression levels between novel transcripts and cDNA defined genes. (E) Organ-specific expression profiles of novel transcripts.
