High-throughput sequence analysis of Ciona intestinalis SL trans-spliced mRNAs: Alternative expression modes and gene function correlates

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Figure 1.
Figure 1.

SL-PCR amplification of 5′ segments of trans-spliced mRNAs. Starting from a capped (m*Gppp) and polyadenylated (AAAAAAAAAAn) trans-spliced mRNA (top line) with the 16-nt SL sequence at its 5′ terminus, the diagram illustrates the basis of AN-linked random-hexamer-primed reverse transcription, SL-primed second strand cDNA synthesis, and PCR amplification with SL and AN primers.

This Article

  1. Published in Advance March 8, 2010, doi: 10.1101/gr.100271.109 Genome Res. 20: 636-645

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