Reproducibility and quantitative ability of LQ-RNAseq. Reproducibility of S. cerevisiae polyA+ RNA (strain DBY746) expression profiles generated in two independent preparations of 250 pg of RNA run on different sequencers (A) and between 250-pg and 400-ng RNA preparations (B). (C,D) Comparison of LQ-RNAseq methodology to the published expression profile generated by oligo(dT) priming of 1 μg of polyA+ RNA from the same yeast strain used in this study (Lipson et al. 2009) (r = 0.915), and by random hexamer priming of 200 ng of RNA from another yeast strain (BY4741), adaptor ligation, and PCR amplification (Nagalakshmi et al. 2008) (r = 0.661). Log10 abundance is expressed using the number of unique reads aligned to each transcript in each sequencing experiment and expressed as reads number per kilobase per 1 million reads.
