Figure 3.
Marks of active and repressed genes in islets. (A) Human islet chromatin enriched for H3K4me1, H3K4me2, H3K4me3, H3K27me3, and control input DNA from four samples was processed and sequenced. Reads were pooled and aligned to the NCBI Genome Build 36.1-hg18, to determine regions that were enriched for binding by modified histones. Note the strong double peak surrounding the transcriptional start site (left end) of ATF4, and the weaker peaks for H3K4me1 and H3K4me2. In B, the HOXB cluster contains significantly enriched regions for H3K27me3 as well as H3K4me3 at several transcription start sites.
